Tag: immune cells

Exploration of the Causal Roles of Immune Cells and Inflammatory Proteins in Aortic Dissection via Mendelian Randomization

Announcing a new article publication for Cardiovascular Innovations and Applications journal. Aortic dissection is a life-threatening condition with complex immunological underpinnings. This article explored the causal relationships among immune cells, inflammatory proteins, and aortic dissection, through Mendelian randomization analysis. A two-step Mendelian randomization approach was used to assess potential mediators, focusing on the roles of blood immune cells and inflammatory proteins.

GWAS data was analyzed for 731 immune cell traits, 91 inflammatory proteins, and aortic dissection. Single-nucleotide polymorphisms were used as instrumental variables, and analyses were conducted with inverse variance weighting and sensitivity tests to ensure robustness.

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Single-Cell RNA Sequencing Maps Immune Cell Heterogeneity in Mice with Allogeneic Cardiac Transplantation

Announcing a new article publication for Cardiovascular Innovations and Applications journal.   Immune cells play important roles in mediating allograft rejection and tolerance after cardiac transplantation. However, immune cell heterogeneity at the single-cell level, and how immune cell states shape transplantation immunity, remain incompletely characterized.

The authors of this article performed single-cell RNA sequencing (scRNA-seq) on immune cells in LNs from a mouse syngeneic and allogeneic cardiac transplantation model. Nine T cell clusters were identified through unsupervised analysis. Pathway enrichment analysis was used to explore the functional differences among cell subpopulations and to characterize the metabolic heterogeneity of T cells.

The transcriptional landscape of immune cells was determined, particularly T cells, and their metabolic transcriptomes in LNs during mouse cardiac transplantation. On the basis of molecular and functional properties, we also identified T cell types associated with transplantation-associated immune processes, including cytotoxic CD8+ T cells, activated conventional CD4+ T cells, and dysfunctional Tregs. The contribution of JunB to the induction of Th17 cell differentiation and restriction of Treg development was further elucidated, and the authors identified that HIF-1a participates in T cell metabolism and function.

The first systematic single-cell analysis of transcriptional variation within the T cell population is presented in this article, providing new insights for the development of novel therapeutic targets for allograft rejection.

https://www.scienceopen.com/hosted-document?doi=10.15212/CVIA.2023.0023

CVIA is available on the ScienceOpen platform and at Cardiovascular Innovations and Applications. Submissions may be made using ScholarOne Manuscripts. There are no author submission or article processing fees. Cardiovascular Innovations and Applications is indexed in the EMBASE, EBSCO, ESCI, OCLC, Primo Central (Ex Libris), Sherpa Romeo, NISC (National Information Services Corporation), DOAJ, Index Copernicus, Research4Life and Ulrich’s web Databases. Follow CVIA on Twitter @CVIA_Journal; or Facebook.

Zhonghua Tong, Ge Mang and Dongni Wang et al. Single-Cell RNA Sequencing Maps Immune Cell Heterogeneity in Mice with Allogeneic Cardiac Transplantation. CVIA. 2023. Vol. 8(1). DOI: 10.15212/CVIA.2023.0023

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