Protective effect of sweroside on heart failure induced by excessive isoproterenol in mice

[GW30-e0006]

Authors: Qing Wang, Huiming Zhang, Congping Su, Hui Luo, Wenchao Jiao, Shuzhen Guo

School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China

OBJECTIVES The aim of this study was to evaluate the protective effect of sweroside on excessive isoproterenol induced heart failure in mice and to explore its target and mechanism.

METHODS Heart failure mice model was established by subcutaneous injection of excessive isoprenaline. Mice were randomly divided into four groups including control group, model group, sweroside group and captopril group. Cardiac function was evaluated by echocardiography, myocardial pathological changes were detected by Hematoxylin and eosin staining (HE staining). Drug targets were predicted by BAT-MAN-TCM database, and protein expression was detected by Western blot.

RESULTS Compared with the control group, the left ventricle ejection fraction (LVEF) and left ventricle fractional shortening (LVFS) of the model group decreased by 37.84 and 48.04% respectively. Left ventricular internal systolic diameter (LVIDs) increased to about 3.06±0.51 mm (1.86±0.59 mm in the control group), with an increase of more than 50%. Compared with the model group, EF of sweroside group increased to 71.83±4.82%, which was close to the control group, FS increased by 71.79%, LVIDs decreased by 30.82%. The above data had statistical significance (P<0.01). In Histopathological examination, thinned left ventricular wall, thinned myocardial fiber, scattered necrosis of myocardial cells, as well as aggregation of inflammatory cells were found in model mice. The morphology of myocardial tissue were obtained approximate normal by sweroside. The BAT-MAN-TCM database suggests that sweroside may play a therapeutic role in heart failure through ATPase Na+/K+Transporting Subunit Alpha1(ATP1A1). Western blot results showed that ATP1A1 protein expression was down-regulated in the model group, and the content of ATP1A1 protein was increased in the sweroside group.

CONCLUSIONS Sweroside can significantly improve the heart failure induced by excessive isoproterenol in mice, and the mechanism may be related to the increase of ATP1A1 protein expression and the improvement of myocardial energy metabolism.