Exosomal circRNA-0008302 from Adipose-derived Stem Cells Protects Against Myocardial Injury

Announcing a new article publication for Cardiovascular Innovations and Applications journal. This study was aimed at investigating the expression and therapeutic potential of circRNAs from adipose-derived stem cell (ADSC) exosomes (ADSC-Exos) for cardiomyocyte injury.

Differentially expressed circRNAs were screened between ADSCs and ADSC-Exos with a circRNA microarray. A differential circRNA-0008302 siRNA plasmid was constructed to obtain ADSC-Exo and ADSC Exommu-circ-0008302 siRNA, respectively; subsequently, M6200 cells were divided into a control group, hydrogen peroxide (H₂O₂) induced group, H₂O₂ + ADSC-Exo group, and H₂O₂ + ADSC-Exo^{mmu-circ-0008302 siRNA} group, and cell viability was evaluated. Apoptosis and intracellular reactive oxygen species were measured. The expression levels of miR-466i-5p were evaluated, and western blotting was performed to detect the expression of methionine sulfoxide reductase A (MsrA) protein.

Expression of circ-0008302 was significantly more elevated in the ADSC-Exo group than the ADSCs group. The treatment protected cardiomyocytes against H₂O₂-induced oxidative injury. Mechanistically, circ-0008302 downregulated miR-466i-5p levels, thus promoting expression of the miR-466i-5p target gene MsrA in cardiomyocytes.

ADSC-Exos play protective roles in mitigating myocardial injury by delivering circ-0008302 to cardiomyocytes; this circRNA targets miR-466i-5p and subsequently enhances the expression of MsrA.

Get full research article at: https://www.scienceopen.com/hosted-document?doi=10.15212/CVIA.2024.0020

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Chengyan Hu, Shuai Wang and Yanling Wang et al. Exosomal circRNA-0008302 from Adipose-derived Stem Cells Protects Against Myocardial Injury. CVIA. 2024. Vol. 9(1). DOI: 10.15212/CVIA.2024.0020